Fumagillin Attenuates Spinal Angiogenesis, Neuroinflammation, and Pain in Neuropathic Rats after Chronic Constriction Injury

Introduction: Angiogenesis in the central nervous system is visible animal models of neuroinflammation and bone cancer pain. However, whether spinal angiogenesis exists contributes to sensitization neuropathic pain remains unclear. This study analyzes impact on Methods: Rats with chronic constriction injury (CCI) sciatic nerve underwent implantation an intrathecal catheter. Fumagillin or vascular endothelial growth factor-A antibody (anti-VEGF-A) was administered intrathecally. Nociceptive behaviors, cytokine immunoassay, Western blot, immunohistochemical analysis assessed effect inhibition CCI-induced Results: VEGF, cluster differentiation 31 (CD31), von Willebrand factor (vWF) expressions increased after CCI ipsilateral lumbar cord compared that contralateral side control rats from post-operative day (POD) 7 28, a peak at POD 14. Tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6 concentrations, but not IL-10 levels, also CCI. anti-VEGF-A reduced thermal hyperalgesia 5 14 mechanical allodynia 3 CCI-upregulated angiogenic factors astrocytes. Furthermore, fumagillin decreased TNF-α amounts levels 14, IL-1β concentrations. Conclusions: significantly ameliorates nociceptive sensitization, angiogenesis, astrocyte activation. Our results suggest inhibitor treatment suppresses peripheral neuropathy-induced neuroinflammation, activation,